Study of plant chemical defense is carried out in less time
Plants are not able to avoid eating on using several chemical defenses for keeping herbivores at the natural harbor. So chemical defenses of the plant are severe for healthy crops and replying to the academic questions related to ecology. Because of the recent techniques for measuring the chemical defenses of plants is time-consuming and need an enormous plant tissue. Whereas the team of Dr. Chandra Jack mentions, a study in Applications in Plant Sciences develops a new technique for studying plant chemical defenses.
A spectrophotometer is an apparatus using traditional methods, defining defense compounds by using light for measuring chemicals in the leaves. The method needs the samples of large tissue, signifying that various leaves will be pooled together and disintegrating expressive variation.
According to Dr. Jack, the biggest constraint is the amount of tissue essential for spectrophotometer-based assays. Because researchers do not combine leaves from a single plant for exploring the systemic response or identify individual plant response. It allows us to measure population-level variation and to parse out the influence of the environment on genes.
Dr. Jack mentions the other limitation of doing an experiment is time. Because huge time and labor are essential for performing assays by using the spectrophotometric method on different samples for measuring. So this controls the convenient design of the experiment and the types of questions required to answer. A rise in demand for time and tissue of the challenges of the traditional method for generating the results or run the sample. Because scientists are capable to carry out the assays normally takes around 40 hours with only 6 hours.
Chemical defense of plants is difficult and includes the generation of many compounds at one time. The conventional techniques require extractions to assay where each class of defense use combined preparation buffers and procedures to extract. So this is practical for measuring the huge range of chemical responses, offering the more specific and high-resolution image of plant defense. Dr. Jack claims that plants generate enzymes and secondary metabolites for herbivore attack, as plant defense is not complete.
So the method describes that the sample preparation buffers are combined and samples are continuously running on a microplate, extensively reducing the expense and time of assaying defense compounds. Dr. Jack commented that “For us, it was a case of necessity being the mother of invention”. “I was planning to set up an experiment and couldn’t find high-throughput assays to accomplish what was needed”. Further, in developing the technique, researchers are opening the avenues of inquiry in plant defenses. Researchers analyze defense compounds, searching for localized and systemic responses, duplicating the findings convenient and compare a large sample of people.